Faculty and Staff Profiles

Chrysanthe Preza
Professor Chair, Electrical Computer Engineering
Email: cpreza@memphis.edu
Office Location: 206 Engr Science Bldg
Education
  • Bachelor of Science with honors (cum laude) Electrical Engineering - Washington University St. Louis - 1987
  • Bachelor of Science with honors (cum laude) Computer Science - Washington University St. Louis - 1987
  • Master of Science (with Thesis) Electrical Engineering - Washington University St. Louis - 1990
  • Master of Science Computer Science - Washington University St. Louis - 1991
  • Doctor of Science Electrical Engineering - Washington University St. Louis - 1998

Work Experience

  • Research Assistant, Biomedical Computer Laboratory - Washington University St. Louis - July 1987 -July 1998
  • Research Associate, Institute for Biomedical Computing - Washington University St. Louis - August 1998 -June 2000
  • Research Associate, Electronic Systems and Signals Research Laboratory, Electrical and Systems Engineering Department - Washington University St. Louis - Sept. 2000 – Aug. 2006
  • Instructor, Electrical and Systems Engineering Department - Washington University St. Louis - Sept. 2000 – Aug. 2006
  • Visiting Assistant Professor, Electrical and Computer Engineering Department - The University of Cyprus - Sept. 2003 – June 2004
  • Adjunct Assistant Professor , School of Biomedical Engineering and Imaging - The University of Tennessee Health Science Center - Aug. 2006-2010
  • Assistant Professor (Secondary Appointment), Dept. of Biomedical Engineering - The University of Memphis - Aug. 2006-Aug. 2010
  • Assistant Professor, Department of Electrical and Computer Engineering - The University of Memphis - Aug. 2006 - Aug. 2010
  • Adjunct Faculty, Orthopedic Surgery and Biomedical Engineering - The University of Tennessee Health Science Center - Sep. 2010 - present
  • Associate Professor, Biomedical Engineering - The University of Memphis - Sep. 2010 - present
  • Associate Professor, Electrical and Computer Engineering - The University of Memphis - Sep. 2010 - present
Honors/Awards
  • Recipient of the 2015 Faudree Professorship - The University of Memphis - 2015 - present
  • Outstanding Faculty Research Award - Herff College of Engineering, The Univ. of Memphis - 2010 & 2015
  • University of Memphis PI Millionaire, 2014 - The University of Memphis - 2014
  • Featured in the OSA’s 2017 Women in Optics Monthly Planner - Optical Society of America - 2017
  • Minorities and Women in Science Interview Series - Optical Society of America - 2012
  • Ring Faculty Award - Herff College of Engineering, The Univ. of Memphis - 2011-2013
  • Outstanding Faculty Research Award - Herff College of Engineering, The Univ. of Memphis - 2010
  • NSF Career Award - National Science Foundation - 2009
  • Best Poster Award - Memphis BioImaging Symposium - 2009
  • Publications Award - Journal of the Optical Society of America - 1999
  • B.S.E.E. cum laude, B.S.C.S. cum laude - Washington University in St. Louis - 1987
  • Member of the Tau Beta Pi Engineering Honorary Society - Washington University St. Louis - 1986
  • Member of the Eta Kappa Nu Engineering Honorary Society - Washington University St. Louis - 1986
  • CASP scholarship: Full tuition and stipend to attend Washington University in St. Louis - Fullbright - 1983-1987

Teaching Experience

  • Computational Methods for Inverse Problems - EECE 7904/8904 - The University of Memphis
  • Professional Development - EECE 7001/8001 - The University of Memphis
  • Fourier Methods in Imaging - EECE 7901/8901 - The University of Memphis
  • Image Processing - EECE 7214/8214 - The University of Memphis
  • Random Signals and Noise - EECE 7251/8251 - The University of Memphis
  • Computational Optical Imaging - EECE 7907/8907 - The University of Memphis
  • Inverse Problems in Imaging- EECE 7904/8904 - The University of Memphis
  • Fourier Optics -EECE 7243/8243 - The University of Memphis
  • Communication Theory - EECE 4231/6231 - The University of Memphis
  • Signals and Systems I - EECE 3203 - The University of Memphis
  • Transform Methods in Network Analysis - EECE 3202 - The University of Memphis
  • Introduction to Electrical Networks - Washington University in St. Louis
  • Electrical and Electronic Circuits Laboratory - Washington University in St. Louis
  • Introduction to Electrical and Computer Engineering - Washington University in St. Louis
  • Signal Analysis for Electronic Systems and Circuits - Washington University in St. Louis
  • Communication Theory and Systems - Washington University in St. Louis
  • Electrical Engineering Senior Design Projects - Washington University in St. Louis
  • Introduction to Electrical and Computer Engineering - The University of Cyprus
  • Introduction to Information Technology - The University of Cyprus

Student Advising/Mentoring

  • Post-doctoral Fellow - Dr. Jing Zheng - 2009
  • Post-doctoral Fellow - Dr. Sharon V. King - 2008
  • Post-doctoral Fellow - Dr. Shuai Yuan - 2008
  • PhD. in Electrical and Computer Enginering - Sreya Ghosh - 2015
  • PhD. in Electrical and Computer Engineering - Nurmohammed Patwary - 2017
  • PhD. in Electrical Engineering - Hasti Shabani - 2017
  • M.S. in Electrical and Computer Engineering - Christopher A. Taylor - 2016
  • M.S. in Electrical Engineering - Nurmohammed Patwary - 2014
  • M.S. in Electrical and Computer Engineering - Matthew J. Bledsoe - May 2014
  • M.S. in Electrical and Computer Engineering - Md. Mizanur Rahman - August 2013
  • M.S. in Electrical Engineering - Gregorio Lobo - Spring 2013
  • M.S. in Biomedical Engineering - Monica Zugravu - May 2012
  • M.S. in Electrical Engineering - Amaradri Mukherjee - May 2012
  • M.S in Electrical Engineering - Sreya Ghosh - Spring 2011
  • M.S. in Electrical Engineering - Vimeetha Myneni - Dec 2009
  • M.S. in Electrical and Computer Engineering - Rakesh K. Duggirala - Dec 2008
  • M.S. in Electrical Engineering - Abhilash K. Challa - Fall 2010

Support

  • “IDBR TYPE A - Improving 3D resolution and reducing sensitivity to spherical aberration in live, thick sample cellular imaging using novel methods in optical sectioning microscopy”, (PI) - National Science Foundation - $750,096.00 - 09/01/14 - 08/31/17
  • "CAREER: Integrated computational optical framework for quantitative space-variant imaging in live-cell fluorescence microscopy" (PI) - National Science Foundation - $791,402.00 - 03/01/09 - 2/28/15 (includes a 1 year no-cost extension)
  • “Collaborative Research: Multimode adaptive 3D microscopy for quantitative analysis of live-cellular dynamic processes in thick samples” (PI) - National Science Foundation - $168,952.00 - 06/01/09 - 05/31/13 (includes a 1 year no-cost extension)
  • “Collaborative Research: Quantitative DIC Microscope for Measuring 3-Dimensional Cell Attributes” (PI) - National Science Foundation - $217,256.00 - 09/01/2005 - 08/31/2009 (includes a year of no-cost extension)
  • “Sensors for Material Identification, Detection and Characterization”, (Research Team Member; S. T. Griffin, PI) - Arkansas State Univ. - $27,000.00 (Funds for C. Preza) - 07/01/10 - 02/19/12
  • “Analysis and Modeling of Nyquist Limited Infrared Detectors” (Research Team Member; E. L. Jacobs, PI) - Northrop Grumman Systems Co. - $16,725.00 (Funds for C. Preza) - 3/2010 – 11/2010

Outreach

  • Girls Experiencing Engineering (GEE) - High school students and teachers and U of M faculty - Summer 2010 & 2012 - Women’s Foundation for a Greater Memphis
  • Herff College Annual E-Day Open Day - Middle and High School students and teachers and U of M faculty - 2009 - present - Herff College of Engineering
  • Award Scholarships for University Education - High School Seniors and Vice Presidents of Education of the Daughters of Penelope - 2007 - 2012 - Local Chapter of the Daughters of Penelope (an international organization, http://www.ahepa.org/dop/)
Consulting
  • Ikonisys, Inc., New Haven, CT - September 2004 - January 2005
Books Published
  • Book Chapter

    Preza, C., King, S. V., Dragomir, N. M., Cogswell, C. J., “Phase Imaging Microscopy – Beyond Darkfield, Phase and Differential Interference Contrast (DIC) Microscopy,” in Handbook of Biomedical Optics, Boas, D. A., Pitris C., and Ramanujam, N., eds., Taylor and Francis Books, 2011. (Invited chapter)

       
Journal Articles
  • Patwary, N., King, S. V., Saavedra, G., and Preza, C., "Reducing impact on aberrations in 3D fluorescence imaging using wavefront coding with a radially symmetric phase mask”, Optics Express, 24(12), 12905-12921, doi: 10.1364/OE.24.012905, 2016.
  • Ghosh, S. and Preza, C., “Three-Dimensional Block-Based Restoration Integrated with Wide-field Fluorescence Microscopy for the Investigation of Thick Specimens with Spatially Variant Refractive Index,” J. Biomed. Opt., 21(4), 046010, doi: 10.1117/1.JBO.21.4.046010, 2016.
  • King, S. V., Doblas, A., Patwary, N., Saavedra, G., Martinez-Corral, M. and Preza, C., “Spatial light modulator phase mask implementation of wavefront encoded 3D computational-optical microscopy”, Applied Optics,”, Applied Optics, 54(29), 8587-8595, doi: 10.1364/AO.54.008587, 2015.
  • Patwary, N., and Preza, C., “Image restoration for three-dimensional fluorescence microscopy using an orthonormal basis for efficient representation of depth-variant point-spread functions”, Biomed. Opt. Express 6(10), 3826-3841, doi: 10.1364/BOE.6.003826, 2015.
  • Ghosh, S. and Preza, C., "Fluorescence Microscopy Point-Spread Function Model Accounting for Aberrations due to Refractive Index Variability within a Specimen," Journal of Biomedical Optics, 20(7), 075003, doi: 10.1117/1.JBO.20.7.075003, 2015.
  • Ghosh, S. and Preza, C., “Characterization of a 3D DH-PSF for fluorescence microscopy in the presence of spherical aberration,” Journal of Biomedical Optics, 18(3):036010, doi: 10.1117/1.JBO.18.3.036010, 2013.
  • Furxhi, O., Jacobs, E. L. and Preza, C., “Image plane coded aperture for Terahertz imaging,” Optical Engineering,51(9), doi:10.1117/1.OE.51.9.091612, 091612-1, 2012.
  • Yuan, S. and Preza, C., “Point-spread function engineering to reduce the impact of spherical aberration on 3D computational fluorescence microscopy imaging,” Optics Express, 19(23), 23298-23314, doi: 10.1364/OE.19.023298, 2011.
  • King, S. V., Libertun, A. R., Piestun, R., Cogswell, C. J., Preza, C., "Quantitative phase microscopy through differential interference imaging," Journal of Biomedical Optics, Vol. 13(2), 024020, doi: 10.1117/1.2907328, 2008.
  • Fox, D. J. Jr., Tysver Velde, H., Preza, C., O’Sullivan, J. A., Smith, W. H., and Woolsey, T. A.,  “Computational hyperspectral interferometry for studies of brain function: proof of concept,”  Applied Optics, Vol. 45, No. 13, pp. 3009-3021, doi: 10.1364/AO.45.003009, 2006.
  • Preza, C. and Conchello, J.-A. “Depth-Variant Maximum-Likelihood Restoration for Three-Dimensional Fluorescence Microscopy,” Journal of the Optical Society of America A, Vol. 21, No. 9, pp. 1593-1601, September, 2004.  
  • Fuhrmann, D. R., Preza, C., O’Sullivan, J. A., Snyder, D. L., and Smith, W.H., “Spectrum Estimation from Quantum-Limited Interferograms,” IEEE Transactions on Signal Processing, Vol. 52, No. 4, pp. 950-961, April, 2004.  
  • Homem, M. R. P., Mascarenhas, N. D. A., Costa, L. F., and Preza C., “Biological Image Restoration in Optical-Sectioning Microscopy Using Prototype Image Constraints,” Real Time Imaging, special issue on Imaging in Bioinformatics, 8(6), 475-490, 2002.  
  • Preza, C., “Rotational‑diversity phase estimation from differential‑interference‑contrast microscopy images, Journal of the Optical Society of America A,17(3), 415‑424, 2000.
  • Preza, C., Snyder, D. L., and Conchello, J.‑A., “Theoretical development and experimental evaluation of imaging models for differential‑interference‑contrast microscopy,” Journal of the Optical Society of America A, 16(9):2185-2199, 1999.
  • McNally, J. G., Preza C., Conchello, J.‑A., and Thomas, Jr., L. J., “Artifacts in Computational Optical‑Sectioning Microscopy,” Journal of the Optical Society of America A,  11(3):1056-1067, 1994.
  • Preza, C., Ollinger, J. M., McNally, J. G., and Thomas, Jr., L. J., “Point‑Spread Sensitivity Analysis for Computational Optical‑Sectioning Microscopy,” Micron and Microscopica Acta, Special issue, 23(4):501‑513, 1992. (Invited paper)
  • Preza, C., Miller, M. I., Thomas, Jr., L. J., and McNally, J. G., “Regularized Linear Method for Reconstruction of Three‑Dimensional Microscopic Objects from Optical Sections,” Journal of the Optical Society of America A, 9(2):219‑228, February 1992.
  • Patwary, N., King, S. V., Shabani Hasti and Preza, C., “Experimental Implementation of Wavefront Encoding in 3D Widefield Fluorescence Microscopy Using a Fabricated Phase Mask Designed to Reduce System Depth Variability,” Imaging and Applied Optics Congress, Computational Optical Sensing and Imaging (COSI), OSA Technical Digest (online) paper CW2D.3, doi: 10.1364/COSI.2016.CW2D.3, 2016.
  • Ghosh, S. and Preza, C., “Block-Based Restoration Method for Wide-field Microscopy of Samples with Variable Refractive Index,” Imaging and Applied Optics Congress, Computational Optical Sensing and Imaging (COSI), OSA Technical Digest (online) paper CM3D.3, doi: 10.1364/COSI.2016.CM3D.3, 2016.
  • Patwary, N. and Preza, C., “Reducing restoration artifacts in 3D computational microscopy using wavefront encoding,” in Computational Imaging XIV, IS&T International Symposium on Electronic Imaging,1-6(6), doi: 10.2352/ISSN.2470-1173.2016.19.COIMG-157, 2016.
  • Shabani, H., Sánchez-Ortiga, E., Preza, C., “Investigating the performance of reconstruction methods used in structured illumination microscopy as a function of the illumination pattern’s modulation frequency,” Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 9713, 971305, doi: 10.1117/12.2213411, 2016.
  • Patwary, N. and Preza, C., “Wavefront encoded computational optical sectioning microscopy reduces depth variability in 3D imaging,” Imaging and Applied Optics Congress, Computational Optical Sensing and Imaging (COSI) Conference, OSA Technical Digest (online) paper CM2E.4, doi:10.1364/COSI.2015.CM2E.4, 2015.
  • King, S. V., Hossain, Md S., and Preza, C., “Dual acquisition of fluorescence and quantitative phase microscopy with high-speed switchable optics for DIC,” Optics in the Life Sciences, OSA Technical Digest (online), Novel Techniques in Microscopy, paper NW3C.4., doi:10.1364/NTM.2015.NW3C.4, 2015.
  • Ghosh, S., and Preza, C., “Space-Variant Image Formation for 3D Fluorescence Microscopy Using a Computationally Efficient Block-Based Model,” Proc. IEEE 12th International Symposium on Biomedical Imaging (ISBI): From Nano to Macro, Paper FrDT4.3, 7163990, 789-792, doi: 10.1109/ISBI.2015.7163990, 2015.
  • Ghosh, S., and Preza, C., “A block-based forward imaging model for improved sample volume representation in computational optical sectioning microscopy,” Proc. SPIE 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII, 93300T, doi:10.1117/12.2077001, 2015.
  • Patwary, N., King, S. V. and Preza, C., “3D microscope imaging robust to restoration artifacts introduced by optically thick specimens,” Proc. SPIE 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII, 93300O, doi:10.1117/12.2077845, 2015.
  • Hossain, Md S., King, S. V. and Preza, C., “An integrated approach to determine prior information for improved wide-field imaging models from computational interference microscopy,” SPIE 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII, 933014, doi:10.1117/12.2077322, 2015.
  • Patwary, N. and Preza, C., “Computationally tractable approach to PCA-based depth-variant PSF representation for 3D microscopy image restoration,” Classical Optics, OSA Technical Digest (online), Computational Optical Sensing and Imaging, paper CW1C.5, Optical Society of America, http://dx.doi.org/10.1364/COSI.2014.CW1C.5, 2014.
  • Preza, C., “Computational imaging for 3D fluorescence microscopy,” Imaging and Applied Optics, OSA Technical Digest (online), Imaging Systems and Applications, paper IW2C, http://dx.doi.org/10.1364/ISA.2014.IW2C.1, 2014.
  • Hossain, Md S., King, S. V. and Preza, C., “Enhanced Extended Depth-of-Field Microscopy via modeling of SLM effects on the applied phase mask,” Imaging and Applied Optics, OSA Technical Digest (online), Imaging Systems and Applications, paper IW4C.4, Optical Society of America, http://dx.doi.org/10.1364/ISA.2014.IW4C.4 , 2014.
  • King, S. V., Doblas, A., Patwary, N., Saavedra, G., Martínez-Corral, M. and Preza, C., “Implementation of PSF Engineering in High-Resolution 3D Microscopy Imaging with a LCoS (Reflective) SLM,” Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, BiOS, SPIE 8949-39, (doi:10.1117/12.2040723), 2014.
  • Doblas, A., King, S. V., Patwary, N., Saavedra, G., Martínez-Corral, M. and Preza, C., “Investigation of the SQUBIC phase mask design for depth-invariant widefield microscopy point-spread function engineering,” Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, BiOS, SPIE 8949-40, (doi:10.1117/12.2038610), 2014.
  • Patwary, N., Doblas, A., King, S. V. and Preza, C., “Reducing depth-induced spherical aberration in 3D widefield fluorescence microscopy by wavefront coding using the SQUBIC phase mask,” Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, BiOS, SPIE 8949-37, (doi:10.1117/12.2040191), 2014.
  • Ghosh, S., Schaefer, L., Schuster, D. and, Preza, C., “Further developments in addressing depth-variant 3D fluorescence microscopy imaging,” Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, BiOS, SPIE 8949-26, 2014.
  • King, S. V. and Preza, C., “Experimental Verification of PSF Engineering Using a Spatial Light Modulator,” OSA Computational Optical Sensing and Imaging (COSI) Conference, Arlington, Virginia, 2013.
  • Yuan, S. and Preza, C., “Computational 3D Microscopy Imaging Using a PCA Depth-Variant PSF Model,” OSA Imaging Systems and Applications (IS) Conference, Arlington, Virginia, 20131. Yuan, S. and Preza, C., “Computational 3D Microscopy Imaging Using a PCA Depth-Variant PSF Model,” OSA Imaging Systems and Applications (IS) Conference, Arlington, Virginia, 2013
  • Rahman, M. M., Schaefer, L., Schuster, D. and Preza, C., “Comparison of computational methods developed to address depth-variant imaging in fluorescence microscopy,” Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, BiOS, SPIE BO402, 2013.
  • King S. V., Yuan, S., Dong, D. and Preza, C., “Reducing the impact of spherical aberration on 3D microscopy imaging using programmable PSF engineering,in Focus on Microscopy, the Netherlands, March 24-27, 2013.
  • Yuan, S. and Preza, C., “Computational optical sectioning microscopy using an engineered PSF with reduced depth variability - proof of concept,” Proc. of the 9th IEEE International Symposium on Biomedical Imaging, 1739 – 1742, 2012.
  • Yuan, S. and C. Preza, “Reducing image artifacts due to depth-induced aberrations in extended depth-of-field microscopy using an engineered point-spread function,” OSA Computational Optical Sensing and Imaging (COSI) Conference, Monterey, California, June 2012.
  • Preza, C., Schaefer, L. H., Schuster, D., Ghaffar, A.-U., Yuan, S., and Lobo, G. J., “Impact of Spherical Aberration on Structured-Illumination Microscopy”, in Focus on Microscopy, Singapore, April 1-4, 2012.
  • Preza, C. and Yuan, S., “Reducing the Impact of Spherical Aberration on Extended depth-of-field Microscopy Using PSF Engineering”, in Focus on Microscopy, Singapore, April 1-4 2012.
  • Rahman, M. M., Abdullah-Al-Wadud, M., and Preza, C., “A Decision-based Filter for Removing Salt-and-Pepper Noise,” International Conference on Informatics, Electronics and Vision (ICIEV12), IEEE/IAPR, Dhaka, Bangladesh, May 18-19, 2012.
  • Ghosh, S., Quirin, S., Grover, G., Piestun, R. and Preza, C., “Computational imaging for fluorescence microscopy using double helix PSF engineering,” Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, BiOS, SPIE 8227, 82270F, 2012.
  • Yuan, S. and C. Preza, “Point-spread function engineering to reduce the impact of depth-induced aberrations on high-NA 3D extended depth-of-field microscopy,” Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, BiOS, SPIE 8227, 822702, 2012.
  • Yuan, S. and C. Preza, “Performance evaluation of an image estimation method based on principal component analysis (PCA) developed for quantitative depth-variant fluorescence microscopy imaging,” Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, BiOS, SPIE 8227, 82270H, 2012.
  • Yuan, S., and Preza, C., “A new approach to reduce depth-introduced aberration in 3-D microscopy”, Memphis BioImaging Symposium (MemBIS), Memphis, 2011.
  • Yuan, S. and C. Preza, “Point-spread function engineering to reduce the impact of depth-induced aberrations on widefield microscopy imaging,” OSA Computational Optical Sensing and Imaging (COSI) Conference, Toronto, July 2011.
  • Ghosh, S. and C. Preza, “Frequency content of the double-helix PSF for 3D microscopy in the presence of spherical aberration,” OSA Computational Optical Sensing and Imaging (COSI) Conference, Toronto, July 2011.
  • Preza, C., Yuan, S., Sporer, M. H., Mukherjee, A., Crosby, L. M., and Waters, C. M., “Investigating the use of structured-illumination microscopy as implemented in the Zeiss ApoTome attachment to image thick lung tissue slices”, in Focus on Microscopy, Konstanz, Germany, April 2011.
  • Preza, C., Ghosh, S., Grover, G., Quirin, S., Piestun, R., “Extraction of depth information in the presence of spherical aberration using double-helix point spread function coding in 3D fluorescence microscopy imaging”, in Focus on Microscopy, Konstanz, Germany, April 2011.
  • Preza, C., “Simulating Structure Illumination Microscopy in the Presence of Spherical Aberrations,” Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, BiOS, SPIE 7904-12, 2011. 
  • Yuan, S. and Preza, C., “3D Fluorescence Microscopy Imaging Accounting for Depth-Varying Point Spread Functions Predicted by a Strata Interpolation Method and a Principal Component Analysis”, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, BiOS, SPIE 7904, 79040D, 2011. 
  • Ghosh, S., Grover, G., Piestun, R. and Preza, C., “Effect of double-helix point-spread functions on 3D imaging in the presence of spherical aberrations,” Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, BiOS, SPIE 7904, 79041D, 2011.
  • Yuan, S., Chen, Y., Preza, C., and Tang, C., “Study on blood-flow pulsation using laser speckle contrast imaging”, Dynamics and Fluctuations in Biomedical Photonics VI, BiOS, SPIE 7898-15, 2011.
  • Myneni, V. and Preza, C., “3D Reconstruction of Fluorescence Microscopy Image Intensities Using Multiple Depth-Variant Point-Spread Functions”, Digital Image Processing and Analysis (DIPA), Imaging and Applied Optics, OSA Optics and Photonics Congress, DTuA2, 2010.
  • Mukherjee, A. and Preza, C., "Computational 3D Fluorescence Microscopy Imaging", OSA Imaging Systems (IS), Imaging and Applied Optics, OSA Optics and Photonics Congress, paper # IWC2, 2010.   
  • Preza, C. and O’Sullivan, J. A., “Implementation and evaluation of a penalized alternating minimization algorithm for computational DIC microscopy”, Computational Imaging VIII, IS&T/SPIE Electronic Imaging, SPIE 7533, pp. 75330E-1 to 75330E-11, 2010.
  • Preza, C. and Myneni, V., “Quantitative depth-variant imaging for fluorescence microscopy using the COSMOS software package,” Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, BiOS, SPIE 7570, pp. 757003-1 to 757003-8, 2010.   
  • Preza, C. and O’Sullivan, J. A., “Computational Differential Interference Contrast (DIC) Microscopy for Quantitative Imaging,” in Computational Optical Sensing and Imaging (COSI), OSA Technical Digest (CD), Optical Society of America, paper # CThB5, 2009.

  • Myneni, V. and Preza, C., “Computational depth-variant imaging for quantitative fluorescence microscopy,” in Computational Optical Sensing and Imaging (COSI), OSA Technical Digest (CD), Optical Society of America, paper # CThC4, 2009.    
  • Myneni, V. and Preza, C., “Computational imaging for depth-variant fluorescence microscopy,” in Focus on Microscopy, Krakow, Poland, p. 164, April 2009.   
  • Preza, C. and O’Sullivan, J. A., “Quantitative phase and amplitude imaging using differential-interference contrast (DIC) microscopy  ”, in Computational Imaging VII, Charles A. Bouman, Eric L. Miller, Ilya Pollak, eds., Electronic Imaging, Proc. SPIE Vol. 7246, 724604, 2009.  
  • Preza, C., King S. V., Libertun, A. R., and Cogswell C. J., “Computational Imaging for DIC Microscopy: Current Use in Live-Cell Imaging”, in Focus on Microscopy, Osaka-Awaji, Japan, April 2008. 
  • O’Sullivan, J. A. and Preza, C., “Alternating Minimization Algorithm for Quantitative Differential-Interference Contrast (DIC) Microscopy”, in Computational Imaging, Charles A. Bouman, Eric L. Miller, Ilya Pollak, eds., Electronic Imaging, Proc. SPIE Vol. 6814, 68140Y, 2008.  
  • Preza, C. and O’Sullivan, J. A., “Quantitative determination of specimen properties using computational differential-interference contrast (DIC) microscopy”, in Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, Tony Wilson, Ammasi Periasam, eds., Proc. SPIE-OSA Biomedical Optics, Proc. SPIE Vol. 6630, 66300E, 2007. 
  • King, S. V., Libertun, A. R., Preza, C., Cogswell, C. J., "Calibration of a phase-shifting DIC microscope for quantitative phase imaging", inThree‑Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, J.‑A. Conchello, C. J. Cogswell, T. Wilson, eds., Proc. SPIE Vol. 6443, 64430M, 2007.
  • Preza, C., King, S. V., and Cogswell, C. J., “Algorithms for extracting true phase from rotationally-diverse and phase-shifted DIC images,” invThree‑Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII, J.‑A. Conchello, C. J. Cogswell, T. Wilson, eds., Proc. SPIE Vol. 6090, 60900E, 2006.
  • Fox, D. J. Jr., Woolsey, T. A., Preza, C., O’Sullivan, J. A., Tysver Velde, H., and Smith, W. H., “Computational Hyperspectral Microscopy for Studies of Neural and Biological Function,” in Adaptive Optics: Analysis and Methods/Computational Optical Sensing and Imaging/Information Photonics/Signal Recovery and Synthesis Topical Meetings on CD-ROM (The Optical Society of America, Washington, DC), presentation # CTuB2, 2005.
  • Preza, C., Patsalis, C. P., Sismani C., Evangelidou P., and Pitris, C., “Three-dimensional FISH Imaging of Chromosomes Using Computational Optical Sectioning Microscopy”, in Diagnostic Imaging and Image Analysis, II Mediterranean Conference on Medical Physics, Limassol, Cyprus, April, 2004.
  • Preza, C. and Conchello, J.‑A., “Image Estimation Accounting for Point-Spread Function Depth-Variation in Three-Dimensional Fluorescence Microscopy,” inThree‑Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X, C. J. Cogswell, J.‑A. Conchello, T. Wilson, eds., Proc. SPIE Vol. 4964, pp. 135-142, 2003.
  • Preza, C., van Munster, E. B., Aten, J. A., Snyder, D. L., and Rosenberger, F. U., “Determination of Direction‑Independent Optical Path‑Length Distribution of Cells Using Rotational‑Diversity Transmitted‑Light Differential Interference Contrast (DIC) Images,” in Three‑Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, C. J. Cogswell, J.‑A. Conchello, T. Wilson, eds., Proc. SPIE 3261A, 60-70, 1998.
  • Preza, C., Snyder, D. L., Rosenberger, F. U., Markham, J., and Conchello, J.‑A., “Phase Estimation from Transmitted‑Light DIC Images Using Rotational Diversity,” in T. J. Schulz, ed., Proc. SPIE 3170, 97‑107, 1997.
  • Preza, C., Snyder, D. L., and Conchello, J.‑A., “Image Reconstruction for Three‑Dimensional Transmitted‑Light DIC Microscopy,” in Three‑Dimensional Microscopy: Image Acquisition and Processing IV, C. J. Cogswell, J.‑A. Conchello, T. Wilson, eds., Proc. SPIE 2984, 220‑231, 1997.
  • Preza, C., Snyder, D. L., and Conchello, J.‑A., “Imaging Models for Three‑Dimensional Transmitted‑Light DIC Microscopy,” In Proceedings of the IS&T/SPIE Symposium on Electronic Imaging: Science & Technology, volume 2655, 245‑257, 1996.
  • Preza, C., Miller, M. I., and Conchello, J.‑A., “Image Reconstruction for 3‑D Light Microscopy with a Regularized Linear Method Incorporating a Smoothness Prior,” Biomedical Image Processing and Biomedical Visualization, R. S. Acharya and D. B. Goldgof, Eds., Proceedings of IS&T/SPIE’s Symposium on Electronic Imaging: Science & Technology, Proc. SPIE 1905:129‑139, 1993.
  • Preza, C., Ollinger, J. M., McNally, J. G., and Thomas, Jr., L. J., “Point‑Spread Sensitivity Analysis for 3‑D Fluorescence Microscopy,” in Biomedical Image Processing and Three‑Dimensional Microscopy, R. S. Acharya, C. J. Cogswell, and D. B. Goldgof, Eds., Proceedings of SPIE, 1660:158‑169, 1992.
  • Preza, C., “Regularized Linear Method for Reconstruction of 3‑D Microscopic Objects from Optical Sections,” Presented at the Optical Society of America Annual Meeting, San Jose, CA, Nov., 1991.
  • Journal Publications under Review/Revision

    King, S. V., Hossein, Md. S., Yuan, S. and Preza C., "Performance of engineered PSFs for Extended Depth of Field Microscopy in the presence of SA evaluated through the computational imaging process", under revision, to be re-submitted 2016.

  • Doblas, A., King, S. V., Patwary, N., Saavedra, G., Martinez-Corral, M. and Preza, C., "Application of SQUBIC PSF design to 3D wide-field fluorescence microscopy reduces depth variability in image formation", under revision, to be re-submitted 2016.
  • Journal Publications Under Preparation

    Preza, C. and O’Sullivan, J. A., “Quantitative phase and amplitude imaging using an alternating minimization algorithm developed for Nomarski differential-interference contrast (DIC) microscopy”, to be submitted to the Journal of Biomedical Optics.

  • Preza, C., Schaefer L. H., Schuster, D. and Ghaffar, A., “Structured Illumination Microscopy in the Presence of Spherical Aberrations,” to be submitted.
  • Shabani, H., Sánchez-Ortiga, E., Preza, C., “Comparison of reconstruction methods used in structured illumination microscopy (review paper),” to be submitted 2016.
  • Preza, C., "Quantitative 3D cell imaging with fluorescence microscopy and the COSMOS software package", to be submitted .  
Presentations
  • Preza, C., “Digital Imaging Principles”, Invited Lecture to be presented at the Optical Microscopy and Imaging in Biomedical Sciences course at the Marine Biology Laboratory, Woods Hole, MA, September 12, 2016.
  • Preza, C., “Advances in Computational imaging for quantitative 3D fluorescence microscopy,” Invited talk presented at the Golden Jubilee Annual Meeting of the Israeli Society for Microscopy (ISM), Haifa, Israel, May 2016. (http://www.ismicroscopy.org.il/ism2016/plenary-speakers/)
  • Preza, C., “Imaging Thick Specimens with Spatially-Variant Refractive Index Using Wide-field Fluorescence Microscopy and Block-Based 3D Image Restoration,” Invited seminar given at the Joint Biomedical Engineering Program of The Univ. of Memphis & The Univ. of Tennessee, Health Science Center, Memphis, Tennessee, February 26, 2016.
  • Preza, C., “Computational imaging for quantitative 3D fluorescence microscopy,” Invited seminar organized by IEEE Kolkata Section and IET(UK) Kolkata Network, Meghnad Saha Institute of Technology, Kolkata, India, December 19, 2015.
  • Preza, C., “Computational imaging for 3D fluorescence microscopy: beyond deconvolution microscopy,” Invited tutorial presentation at the Quantitative BioImaging 2015, Institut Pasteur, Paris, France, 2015. (http://www.quantitativebioimaging.com/program/talks/)
  • Preza, C., “Computational imaging for 3D fluorescence microscopy,” Invited talk at Imaging Systems and Applications, of the Imaging and Applied Optics Congress, OSA, Seattle, 2014.
  • Preza, C., “Computational Imaging for 3D Fluorescence Microscopy,” Invited seminar given at the Seminar Series of the Physics Dept., Rhodes College, January 24, 2014.
  • Preza, C., “3D point spread function characterization for widefield fluorescence microscopy,” Invited tutorial presentation at the Quantitative BioImaging 2013, University of New Mexico, Albuquerque, New Mexico, 2013.
  • Preza, C., “Advances in 3D Computational Imaging for Structured-Illumination Microscopy,” Invited seminar given at the Joint Biomedical Engineering Program of The Univ. of Memphis & The Univ. of Tennessee, Health Science Center, Memphis, Tennessee, September 14, 2012.
  • Preza, C., “3D Fluorescence Microscopy Imaging Using Double-Helix Point-Spread Function Engineering,” Invited seminar given at the Optical Fibers and Signal Processing Group, The Univ. of Valencia, Spain, July 5, 2012.
  • Preza, C., “New Computational Methods for Structured-Illumination Microscopy,” Invited seminar given at the Dept. of Optics, The Univ. of Valencia, Spain, April 30, 2012.
  • Preza, C., “Quantitative 3D Fluorescence Microscopy Imaging Using Computational Methods,” Invited seminar given at the Seminar Series of the Dept. of Biological Sciences, The Univ. of Memphis, Memphis, November 10, 2011.
  • Preza, C., “3D Fluorescence Microscopy Imaging Using Double-Helix Point-Spread Function Engineering,” Invited seminar given at the Optical Fibers and Signal Processing Group, The Univ. of Valencia, Spain, July 5, 2012.
  •  Preza, C., “Correction of Depth-Induced Aberrations in 3D Fluorescence Microscopy Imaging,” Invited seminar given at the Seminar Series of the Physics Dept., The Univ. of Mississippi, Oxford, April 26, 2011.
  • Preza, C., “Correction of Depth-Induced Aberrations in 3D Fluorescence Microscopy Imaging,” Invited seminar given at the Seminar Series of the Department of Electrical and Computer Engineering, Purdue School of Engineering and Technology, Indiana University / Purdue University at Indianapolis, December 9, 2010.
  • Preza, C., “Correction of Depth-Induced Aberrations in 3D Fluorescence Microscopy Imaging,” Invited seminar given at the Seminar Series of the Department of Electrical and Computer Engineering, The University of Alabama, Tuscaloosa, Alabama, November 19, 2010.
  • Preza, C., “Correction of Depth-Induced Aberrations in 3D Fluorescence Microscopy Imaging,” Invited seminar given at the Seminar Series of the Physics Dept., The Univ. of Memphis, Memphis, October 14, 2010.  
  • Preza, C., “Alternating Minimization Algorithm for Quantitative Differential-Interference Contrast (DIC) Microscopy,” Invited seminar given at the Computational Optical Imaging and Sensing Seminar Series of the Electrical & Comp. Eng.  Dept., Univ. of Colorado, Boulder, Colorado, October 13, 2008. 
  • Preza, C., “Computational Imaging for Microscopy,” Invited seminar given at the Chemical Engineering Departmental Seminar Series, Tennessee Technological University, Cookeville, Tennessee, September 30, 2008. 
  • Preza, C., “Computational Optical Sectioning Microscopy,” Invited seminar given at the Electrical & Comp. Eng.  Departmental Seminar Series, Univ. of Colorado, Boulder, Colorado, March 6, 2007. 
  • Preza, C., “Computational Imaging for Microscopy,” Invited seminar given at the Joint Biomedical Engineering Program of The University of Memphis and The University of  Tennessee, University of Tennessee Health Science Center, Memphis, Tennessee, September 8, 2006.
  • Preza, C., “Computational Imaging for Depth-Varying Fluorescence Microscopy,” Invited seminar given at CDM Optics, Inc., Boulder, Colorado, March 17, 2006.
  • Preza, C., “Computational Imaging for Microscopy,” Invited seminar given at the Electrical and Computer Engineering Department, University of Colorado, Boulder, Colorado, March 14, 2006.
  • Preza, C., “Computational Imaging for Microscopy,” Invited seminar given at the Electrical and Computer Engineering Department, Michigan Technological University, Houghton, Michigan, February 16, 2006.
  • Preza, C., “Computational Imaging for Microscopy,” Invited seminar given at the Electrical and Computer Engineering Department, Texas A&M University, College Station, Texas, Nov. 4, 2005.
  • Preza, C., “Computational Imaging for Fluorescence Microscopy,” Invited seminar given at Ikonisys, Inc., New Haven, Connecticut, July 9, 2004.
  • Preza, C., “Computational Imaging for Microscopy,” Invited seminar given at the Electrical and Computer Engineering Department, Purdue University, West Lafayette, Indianapolis, May 5, 2003.
  • Preza, C., “Computational Imaging for Microscopy,” Invited seminar given at the Electrical and Computer Engineering Department, University of Cyprus, Nicosia, Cyprus, March, 2003.
  • Preza, C., “Progress in Quantitative Differential‑Interference‑Contrast Microscopy Using Rotational‑Diversity Phase Estimation,” presented at The Donald L. Snyder Workshop, Washington University, St. Louis, MO, January 15, 2000.
  • Preza, C., “Phase Estimation Using Rotational Diversity for Differential Interference Contrast Microscopy, Invited seminar given at the Electrical, Computer, & Systems Engineering Department, Rensselaer Polytechnic Institute, Troy, New York, February 26, 1998.
  • Preza, C., “Computational Microscopy,” Invited seminar given at The Cyprus Institute of Neurology and Genetics, Nicosia, Cyprus, October 23, 1997.
  • Preza, C., “Imaging Models for Transmitted‑Light DIC Microscopy”, Invited seminar given at the Center for Light Microscope Imaging and Biotechnology , Carnegie Mellon University, Pittsburgh, Pennsylvania, July 10, 1996.
  • Preza, C., “Comparison of Image Restoration Algorithms,” Invited presentation given at the IBC’s Tutorial on Image Restoration for Three‑Dimensional Microscopy, Institute for Biomedical Computing, Washington University, St. Louis, MO, April 26‑27, 1996.
  • Preza, C., “Regularized Linear Method for Computational Optical‑Sectioning Microscopy,” Invited seminar given at the Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, Pennsylvania, April 2, 1992.
Appendix C - Research/Scholarship/Creative Activities
  • Research Projects in my lab (CIRL)
  • 1)  Improving 3D resolution and reducing sensitivity to spherical aberration in live, thick sample cellular imaging using novel methods in optical sectioning microscopy (funded by NSF). PI: C. Preza; co-PI: O. Skalli, Univ. of Memphis; Collaborators: G. Saavedra and M. Martinez-Coral, Univ. of Valencia, Spain; C. Waters, Univ. of Tennessee Health Science Center. This project  is aims to develop a robust and flexible computational optical system with improved 3D resolution and reduced sensitivity to depth-induced aberrations that will greatly extend the capabilities of optical-sectioning microscopy for use in a wide range of biological applications. 

  • 2) Integrated computational optical framework for quantitative space-variant imaging in live-cell fluorescence microscopy (Funded by the NSF). As part of this NSF CAREER project, I have developed a new theoretical framework that will facilitate the development of new imaging methodologies such as an enhanced fluorescence microscope (proposed in our recently funded NSF IDBR proposal discussed in project (2) below) and a software package (COSMOS) suitable for processing data from live cell imaging of thick samples. The research mainly focuses on 1) the development of new algorithms suitable for processing data from multiple current and future microscope system designs; and 2) algorithm fine tuning to address specific needs of a variety of biological applications (i.e. studies of epithelial wound healing mechanisms and morphodynamic changes in cancer cells). It is anticipated that this framework will result in a fundamentally new approach that has the potential to change the way all data acquired with microscopes are processed. The new computational methods are integrated with a novel optical non-scanning imaging system design to enable live-cell fluorescence microscopy imaging with high three-dimensional (3D) resolution.  This work addresses a key challenge in live-cell microscopy by accounting for aberrations due to “thick” biological specimens. The computational methods are applied to biological applications that are currently limited by the available imaging methodologies and are disseminated to biological users in an open source software package (COSMOS). The COSMOS package is available for a free download from my website http://cirl.memphis.edu/cosmos/ . The Computational Optical Sectioning Microscopy Open Source (COSMOS) package has four platform-independent graphical user interfaces (developed using a visualization tool kit) that facilitate point-spread function generation, image intensity estimation (aka “deconvolution”, for the computation of quantitative image intensity from measured data), image visualization, and performance analysis of our algorithms. Due to the success of deconvolution microscopy,  the COSMOS software has a broad impact.

  • 3) Development of a multimode adaptive 3D microscope for quantitative analysis of live-cellular dynamic processes in thick samples (funded with an NSF IDBR award). In collaboration with investigators at the University of Colorado, Boulder we investigated and developed novel approaches that integrate optics and computations to enhance the capabilities and 3D resolution of  a multimode microscope that will allow simultaneous fluorescence and phase imaging of live-cellular dynamic processes in thick samples that might otherwise go undetected. The research focused on integrating side-illumination and correction  of aberrations using adaptive optics and depth from defocus approaches.  
  • 4) Quantitative Imaging with Computational DIC Microscopy (Funded by the NSF). In collaboration with investigators at the University of Colorado, Boulder we have been developing and evaluating an enhanced computational Differential Interference Contrast (DIC) microscope for the visualization and measurement of dynamic processes in living cells.  My role in the project is leading the diffraction model-based algorithm development for computation of quantitative specimen phase from DIC images. Additionally, in collaboration with J. A. O’Sullivan (Washington Univ.) our methodology has been extended to address practical issues such as the absorption of semi-transparent specimens resulting in new computational methods that provide simultaneous quantitative phase and amplitude imaging from DIC images. The goal of the research has been to develop rigorous and practical methods that are well suited for real applications and yield meaningful and useful results. The methods are developed to satisfy specific constraints and limitations while exploiting the information available in the measured data. Performance of the methods is thoroughly tested with simulations. Calibration and utility of the methods and the computational DIC microscope is tested with data from various biological applications.

  • Collaborators:
  • Current
  • Jason Braasch – The University of Memphis

  • Sharon V. King – The University of Memphis

  • Manuel Martinez-Coral – University of Valencia, Spain

  • Joseph A. O’Sullivan – Washington University

  • Genaro Saavedra – University of Valencia, Spain

  • Lutz H. Schaefer – Advanced Imaging Methodology Consultation, Canada

  • Dietwald Schuster - Hochschule Regensburg - University of Applied Sciences

  • Omar Skalli – The University of Memphis

  • Deborah Tollefsen – The University of Memphis

  • Christopher Waters – Univ. of Tennessee Health Science Center

  • Past
  • Carol J. Cogswell – University of Colorado at Boulder
  • Jose-Angel Conchello – Harvard University Center for Brain Science
  • Nicoleta Dragomir –Victoria University, Australia
  • Daniel R. Fuhrmann – Michican Tech
  • Orges Furxhi – St. Johns Optical Systems
  • Steven T. Griffin – The University of Memphis
  • Khan M. Iftekharuddin – Old Dominion University
  • Stephanie Ivey –The Univ. of Memphis
  • Eddie Jacobs – The Univ. of Memphis
  • Robert Kozma –The Univ. of Memphis
  • James G. McNally – Helmholtz-Zentrum Berlin, Germany
  • Michael I. Miller – Johns Hopkins University
  • John Ollinger – Walter Reed National Military Medical Center
  • Rafael Piestun – University of Colorado at Boulder
  • Aaron Robinson –The Univ. of Memphis
  • Donald L. Snyder – Washington University
  • William H. Smith – Washington University
  • Lan Wang –The Univ. of Memphis
  • Thomas A. Woolsey – Washington University